The former first-round pick’s agent, Brian Overstreet, confirmed Gladney’s death to the Fort Worth Star-Telegram.
“We are asking prayers for the family and privacy at this most difficult time,” Overstreet said in a statement.
A 26-year-old female passenger was also killed.
Gladney was drafted by the Minnesota Vikings in 2020 out of TCU.
He was charged with assaulting his former girlfriend in April 2021 and released several months later.
Gladney was found not guilty in March 2022 and signed with the Cardinals soon after.
“We are devastated to learn Jeff Gladney’s passing,” the Cardinals said in a statement. “Our hearts go out to his family, friends and all who are mourning this tremendous loss.”